In 2015, Caribbean nations were overwhelmed by an unprecedented quantity of Sargassum that washed ashore. This issue prompted international discussion to better understand the origin, distribution, and movement of Sargassum, a free-floating brown seaweed with ecological, environmental, and economic importance. In the open ocean, Sargassum mats serve a vital ecological function. However, when large quantities appear onshore without warning, Sargassum threatens local tourist industries and near-shore ecosystems. As part of the international response, this project investigated the proliferation of this seaweed using National Aeronautics and Space Administration (NASA) Earth observations for detection of Sargassum and available nutrients across the region. The NASA DEVELOP National Program Caribbean Oceans team at the NASA Ames Research Center compared Landsat 8 Operational Land Imager (OLI) imagery to Aqua Moderate Resolution Imaging Spectroradiometer (MODIS) and Sentinel II MultiSpectral Instrument (MSI) imagery across a number of indices for the identification of Sargassum. The presence of Sargassum was then compared to ground truth data points from Sea Education Association cruises and social media platforms, as well as various oceanic variables, to determine the ideal pelagic environment for Sargassum growth. As part of the international effort to better understand the life cycle of Sargassum in the Caribbean, the results of this project will assist local economies and help promote sustainable management practices.